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Mesquite Weekend Follow-up: Oyster Mushroom Workshop

Hi there! Welcome to my website! If you’re looking for more information on the Mesquite Weekend Oyster Mushroom Workshop then you are in the right place! If you just found this page otherwise, I recently hosted a workshop in which participants combined ingredients to create ready-to-go oyster mushroom grow bags. This is the recipe for the exact grow bags that we made in the workshop.

If this is all new to you, here’s a list of key terms to help:

  • Mycelium – An interconnected mosaic of thin filaments called hyphae that collect food, water, and nutrients to nourish the fungus
  • Fruiting Body – the spore-producing organ of a fungus, often seen as a mushroom or toadstool.
  • Substrate – a medium that allows mushroom mycelium to develop and establish itself
  • Spawn – Mycelium colonized over a substrate that is used to further inoculate more substrate
  • Fruiting Conditions – creating an environment of high humidity and more fresh air exchange to promote the fruiting stage of the mushroom

The recipe for each grow bag is as follows:

Non-Sterile Oyster Mushroom Grow Bag
Using a 1 Gallon Ziplock bag, add:
4 cups Pelletized newspaper
¼ cup rabbit/guinea pig food
4 cups water
> > > Mix everything up good and wait a few minutes for absorption
Add ¼ cup spawn (we used colonized grain spawn)
Mix thoroughly and seal bag shut (without pushing the excess air out)
A fully colonized block of Oyster Mushroom spawn

You’re almost there! Once you mix it all up, you just need to place your grow bag out of direct sunlight at room temperature and wait 2-4 weeks for the mycelium to colonize the substrate. Once the mycelium appears to have colonized all of the substrate (see image above), folks can initiate fruiting by pushing any air out of the bag, resealing and cutting a small “X” in the bag. Oyster mushrooms are naturally a side-fruiting mushroom, growing horizontally from the mycelium. For best fruiting results, I’d recommend standing your bag up and cutting the slit in the side of the bag.

While in fruiting conditions, mushrooms require high humidity to retain the moisture in the fruiting bodies. To make a more humid environment for your mushies, you can make a small tent using a plastic grocery bag and a few sticks/paint stirrers to support the grocery bag up off of the grow bag. Spray water in the grocery bag with a mister every day or so to keep the tent humid.

That’s pretty much all there is to it!

If you want to go a step further, you can create a Shotgun Fruiting Chamber which holds a steady humidity a little better than a plastic bag and looks much nicer as well. They’re pretty easy to make, all you do is get a plastic tote with some holes drilled in it for air flow, add about 1″ of perlite in the bottom, soak the perlite with water, and place your ready-to-fruit grow bag inside the tote and place the lid on!

A Simple Shotgun Fruiting Chamber (SGFC)
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